Posted by: reganenosusd | September 9, 2014

July 2014

Huber, Victor C. (2014). Influenza vaccines: from whole virus preparations to recombinant protein technology. Expert Review of Vaccines, 13(1), 31-42.

Vaccination against influenza represents our most effective form of prevention. Historical approaches toward vaccine creation and production have yielded highly effective vaccines that are safe and immunogenic. Despite their effectiveness, these historical approaches do not allow for the incorporation of changes into the vaccine in a timely manner. In 2013, a recombinant protein-based vaccine that induces immunity toward the influenza virus hemagglutinin was approved for use in the USA. This vaccine represents the first approved vaccine formulation that does not require an influenza virus intermediate for production. This review presents a brief history of influenza vaccines, with insight into the potential future application of vaccines generated using recombinant technology.

Basic Biomedical Sciences, Vermillion Campus.

Liu, N. N., Liu, C. J., Li, X. F., …., Wang, Xuejun, Dou, Q. P., & Liu, J. B. (2014). A novel proteasome inhibitor suppresses tumor growth via targeting both 19S proteasome deubiquitinases and 20S proteolytic peptidases. Scientific Reports, 4, 13.

The successful development of bortezomib-based therapy for treatment of multiple myeloma has established proteasome inhibition as an effective therapeutic strategy, and both 20S proteasome peptidases and 19S deubiquitinases (DUBs) are becoming attractive targets of cancer therapy. It has been reported that metal complexes, such as copper complexes, inhibit tumor proteasome. However, the involved mechanism of action has not been fully characterized. Here we report that (i) copper pyrithione (CuPT), an alternative to tributyltin for antifouling paint biocides, inhibits the ubiquitin-proteasome system (UPS) via targeting both 19S proteasome-specific DUBs and 20S proteolytic peptidases with a mechanism distinct from that of the FDA-approved proteasome inhibitor bortezomib; (ii) CuPT potently inhibits proteasome-specific UCHL5 and USP14 activities; (iii) CuPT inhibits tumor growth in vivo and induces cytotoxicity in vitro and ex vivo. This study uncovers a novel class of dual inhibitors of DUBs and proteasome and suggests a potential clinical strategy for cancer therapy.

Basic Biomedical Sciences, Vermillion Campus.

Ouellette, Scott P., Rueden, Kelsey J., Gauliard, E., Persons, L., de Boer, P. A., & Ladant, D. (2014). Analysis of MreB interactors in Chlamydia reveals a RodZ homolog but fails to detect an interaction with MraY. Frontiers in Microbiology, 5.

Chlamydia is an obligate intracellular bacterial pathogen that has significantly reduced its genome in adapting to the intracellular environment. One class of genes for which the bacterium has few annotated examples is cell division, and Chlamydia lacks FtsZ, a central coordinator of the division apparatus. We have previously implicated MreB as a potential substitute for FtsZ in Chlamydia (Ouellette et al., 2012). Thus, to identify new chlamydial cell division components, we searched for proteins that interacted with MreB. We performed a small-scale screen using a Gateway (R) compatible version of the Bacterial Adenylate Cyclase Two Hybrid (BACTH) system, BACTH(Gw), to detect proteins interacting with chlamydial MreB and identified a RodZ (YfgA) homolog. The chlamydial RodZ aligns well with the cytoplasmic domain of E. coli RodZ but lacks the periplasmic domain that is dispensable for rod cell shape maintenance in E. coli. The expression pattern of yfgA/rodZ was similar to that of mreB and ftsl, suggesting that these genes may operate in a common functional pathway. The chlamydial RodZ correctly localized to the membrane of E. coli but was unable to complement an E. coli rodZ mutant strain, likely because of the inability of chlamydial RodZ to interact with the native E. coli MreB. Finally, we also tested whether chlamydial MreB could interact with MraY, as suggested by Gaballah et al. (2011). However, we did not detect an interaction between these proteins even when using an implementation of the BACTH system to allow native orientation of the N- and C-termini of MraY in the periplasm. Thus, further work will be needed to establish this proposed interaction. In sum, we have added to the repertoire of potential cell division proteins of Chlamydia.

Basic Biomedical Sciences, Vermillion Campus.

Shewchuk, Richard M., Carlson, Greg L., Klosterman, Matt, Cullen, Stephen, & Qu, Haiyan. (2014). Frictions as Barriers to Perioperative Alignment: Results From a Latent Class Analysis. Quality Management in Health Care, 23(3), 188-200.

The quality of the relationship between the sterile processing department (SPD) and the operating room (OR) is an important determinant of OR safety and performance. In this article, the concept of “friction ” refers to the SPD behaviors and attributes that can negatively affect OR performance. Panels of SPD professionals initially were asked to identify and operationally define different ways in which behaviors of a hospital’s SPD could compromise OR performance. A national convenience sample of OR nurses (N = 291 ) rated 14 frictions in terms of their agreement or disagreement that each had a negative effect on OR performance in their hospital. Overall, more than 50% of the entire sample agreed that 2 frictions, “SPD does not communicate effectively with the OR” (55%) and “SPD inventories are insufficient for surgical volume” (52%), had negative effect on OR performance. However, a latent class analysis revealed 3 distinct classes of nurses who varied with respect to their level of agreement that SPD-OR frictions negatively affected OR performance. The observed heterogeneity in how different groups of nurses viewed different frictions suggests that effective efforts aimed at reducing performance-limiting frictions should be customized so that resources can be used where they are most needed. [ABSTRACT FROM AUTHOR]

Copyright of Quality Management in Health Care is the property of Lippincott Williams & Wilkins — Nursing and

Beacom School of Business.

Thiruppathi, Eagappanath, & Mani, Gopinath. (2014). Vitamin-C Delivery from CoCr Alloy Surfaces Using Polymer-Free and Polymer-Based Platforms For Cardiovascular Stent Applications. Langmuir, 30(21), 6237-6249.

Antiproliferative drugs such as paclitaxel and sirolimus are delivered from stents to inhibit the growth of smooth muscle cells (SMCs) for preventing neointimal hyperplasia. However, these drugs delay the growth of endothelial cells (ECs) as well and cause late stent thrombosis. We recently demonstrated the use of Vitamin-C (L-ascorbic acid, L-AA) over paclitaxel and sirolimus for inhibiting SMCs growth and promoting EC growth simultaneously. In this study, we have investigated the delivery of L-AA from CoCr alloy surfaces for potential use in stents. A polymer-free phosphoric acid (PA) platform and a polymer-based poly-(lactic-co-glycolic acid) (PLGA) platform were used for coating L-AA onto CoCr surfaces. For the PA platform, FTIR confirmed that the PA was coated on CoCr, while the AFM showed that the PA coating on the CoCr surface was homogeneous. The successful deposition of L-AA on PA-coated CoCr was also confirmed by FTIR. The uniform distribution of L-AA crystals on PA-coated CoCr was shown by SEM, optical profilometer, and AFM. The drug release studies showed that L-AA (276 mu g/cm(2)) was burst released from the PA platform by 1 h. For the PLGA platform, SEM showed that the L-AA incorporated polymer films were smoothly and uniformly coated on CoCr. FTIR showed that L-AA was incorporated into the bulk of the PLGA film. DSC showed that the L-AA was present in an amorphous form and formed an intermolecular bonding interaction with PLGA. The drug release studies showed that L-AA was sustained released from the PLGA coated CoCr for up to 24 h. The SEM, FTIR, and DSC characterizations of samples collected post drug release shed light on the mechanism of L-AA release from PLGA coated CoCr. Thus, this study demonstrated the delivery of L-AA from biomaterial surfaces for potential applications in stents and other implantable medical devices.

Biomedical Engineering, Sioux Falls Campus.

Dong, F., Koodali, Ranjit T., Wang, H. Q., & Ho, W. K. (2014). Nanomaterials for Environmental Applications. Journal of Nanomaterials.

Chemistry Department.

Han, Yulun, Lin, Cuikun,Meng, Qingguo, Dai, Fenggrong, Sykes, Andrew G., Berry, Mary T., & May, P. Stanley. (2014). (BMI)(3)LnCl(6) Crystals as Models for the Coordination Environment of LnCl(3) (Ln = Sm, Eu, Dy, Er, Yb) in 1-Butyl-3-methylimidazolium Chloride Ionic-Liquid Solution. Inorganic Chemistry, 53(11), 5494-5501.

A series of (BMI)(3)LnCl(6) (Ln = Sm, Eu, Dy, Er, Yb) crystals was prepared from solutions of LnCl(3) dissolved in the ionic liquid, 1-butyl-3-methylimidazolium chloride (BMICl). Crystals with Ln = 5% Sm + 95% Gd and with Ln = 5% Dy + 95% Gd were also grown to assess the importance of cross-relaxation in the Sm and Dy samples. The crystals are isostructural, with monoclinic space group P2(1)/c and four formula units per unit cell. The first coordination sphere of Ln(3+) consists of six Cl- anions forming a slightly distorted octahedral LnCl(6)(3-) center. The second coordination sphere is composed of nine BMI+ cations. The emission spectra and luminescence lifetimes of both (BMI)(3)LnCl(6) crystals and LnCl(3) in BMICl solution were measured. The spectroscopic similarities suggest that crystalline (BMI)(3)LnCl(6) provides a good model of the Ln(3+) coordination environment in BMICl solution.

Chemistry Department.

Jia, L., Hui, Y. C., Li, Z. S., Sun, H. L., & Wang, Zhenqiang. (2014). Luminescent lanthanide-2-phenylpyrimidine-carboxylate frameworks: structure and luminescence tuning. Crystengcomm, 16(28), 6483-6490.

Six novel luminescent lanthanide-2-phenylpyrimidine-carboxylate frameworks, namely, [Ln(ppmc)(3)(phen)] (Ln = Eu (1), Tb (2)), [Ln(ppmc)(2)(C2O4)(0.5)(H2O)] (Ln = Eu (3), Tb (4)), {[Eu(ppmdc)(phen)(C2O4)(0.5)]center dot 0.5H(2)O} (5) and [Tb(ppmdc)(benzoate)(phen)] (6) (Hppmc = 2- phenylpyrimidine-4-carboxylic acid, H(2)ppmdc = 2-phenylpyrimidine-4,6-dicarboxylic acid) have been synthesized. The X-ray structure analyses reveal that all of the compounds contain carboxylate-bridged dimer units; however, coordination environments of Ln(3+) ions, bridging modes of carboxylate, and the linkage between them are different. In the isostructural compounds 1 and 2, extensive pi-pi stacking exists between the anti-anti carboxylate bridged dimer. However, syn-anti carboxylate bridges are found in compounds 3 and 4 in which oxalate connects the dimers to form 1D chains. Compound 5 has a 2D structure connected by both mu 4-ppmdc(2-) and oxalate in which the carboxylates adopt both anti-anti and chelate-bridging modes. Compound 6 featuring 1D chains also contains anti-anti carboxylate-bridged dimers that are linked by the mu 3-ppmdc(2-) ligand. All six compounds show characteristic red or green emission attributed to Eu3+ and Tb3+. The luminescence lifetime and quantum yield are presented and discussed in detail. The use of different ligands and reaction conditions has led to distinct coordination environments of lanthanide ions and the aggregation of dimeric units, which are considered as the dominating factors for the resulting photoluminescence behaviour of solid samples.

Chemistry Department.

Rasalingam, Shivatharsiny, Peng, Rui, & Koodali, Ranjit T. (2014). Removal of Hazardous Pollutants from Wastewaters: Applications of TiO2-SiO2 Mixed Oxide Materials. Journal of Nanomaterials.

The direct release of untreated wastewaters from various industries and households results in the release of toxic pollutants to the aquatic environment. Advanced oxidation processes (AOP) have gained wide attention owing to the prospect of complete mineralization of nonbiodegradable organic substances to environmentally innocuous products by chemical oxidation. In particular, heterogeneous photocatalysis has been demonstrated to have tremendous promise in water purification and treatment of several pollutant materials that include naturally occurring toxins, pesticides, and other deleterious contaminants. In this work, we have reviewed the different removal techniques that have been employed for water purification. In particular, the application of TiO2-SiO2 binary mixed oxide materials for wastewater treatment is explained herein, and it is evident from the literature survey that these mixed oxide materials have enhanced abilities to remove a wide variety of pollutants.

Chemistry Department.

Suter, John D., Pekas, Nicholas J., Berry, Mary T., & May, P. Stanley. (2014). Real-Time-Monitoring of the Synthesis of beta-NaYF4:17% Yb,3% Er Nanocrystals Using NIR-to-Visible Upconversion Luminescence. Journal of Physical Chemistry C, 118(24), 13238-13247.

In situ real-time monitoring of upconversion emission is applied to study the reaction mechanism for the synthesis of beta-NaYF4:17% Yb,3% Er nanoparticles in oleic acid and octadecene via the heat-up method. Transmission electron microscopy is used to correlate the spectroscopic signature of the reaction mixture with its composition. The power of real-time spectroscopic monitoring to precisely time the duration of the various stages of the reaction, and to accurately identify the transitions between those stages, including the completion of the reaction, is demonstrated. During the heat-up stage, the initial precipitate present is transformed into small alpha-phase nanoparticles. In the second and longest stage of the reaction, a period of relative stasis is maintained, during which there is at most a slight change in the size distribution of the alpha-phase nanoparticles formed during heat-up. In the third stage, a relatively rapid conversion of small alpha-phase nanoparticles to the larger beta-phase product nanoparticles is observed. The size distribution of the beta product remains relatively constant during the phase-transition stage, indicating that, once the beta particles begin to form, they grow very rapidly to the final product size. A significant variation in the time required to complete the reaction is observed, which is due almost entirely to the variation in the duration of the second reaction stage. The time required to complete the reaction does not appear to affect the size or uniformity of the product beta-NaYF4:17% Yb,3% Er nanoparticles.

Chemistry Department.

Wang, C. Y., Lin, Cuikun, Zhang, L. H., Quan, Z. W., Sun, K., Zhao, Bo, . . . Fang, J. Y. (2014). Pt3Co Concave Nanocubes: Synthesis, Formation Understanding, and Enhanced Catalytic Activity toward Hydrogenation of Styrene. Chemistry-a European Journal, 20(6), 1753-1759.

We report a facile synthesis route to prepare high-quality Pt3Co nanocubes with a concave structure, and further demonstrate that these concave Pt3Co nanocubes are terminated with high-index crystal facets. The success of this preparation is highly dependent on an appropriate nucleation process with a successively anisotropic overgrowth and a preservation of the resultant high-index planes by control binding of oleyl-amine/oleic acid with a fine-tuned composition. Using a hydrogenation of styrene as a model reaction, these Pt3Co concave nanocubes as a new class of nanocatalysts with more open structure and active atomic sites located on their high-index crystallographic planes exhibit an enhanced catalytic activity in comparison with low-indexed surface terminated Pt3Co nanocubes in similar size.

Chemistry Department.

Yulun, Han, Cuikun, Lin,Qingguo, Meng, Dai, Fengrong, Sykes, Andrew G., Berry, Mary T., & May, P. Stanley. (2014). (BMI)3LnCl6 Crystals as Models for the Coordination Environment of LnCl3 (Ln = Sm, Eu, Dy, Er, Yb) in l-Butyl-3-methylimidazolium Chloride Ionic-Liquid Solution. Inorganic Chemistry, 53(11), 5494-5501.

A series of (BMI)3LnCl6 (Ln = Sm, Eu, Dy, Er, Yb) crystals was prepared from solutions of LnCl3 dissolved in the ionic liquid, l-butyl-3-methylimidazolium chloride (BMICl). Crystals with Ln = 5% Sm + 95% Gd and with Ln = 5% Dy + 95% Gd were also grown to assess the importance of cross-relaxation in the Sm and Dy samples. The crystals are isostructural, with monoclinic space group P2l/c and four formula units per unit cell. The first coordination sphere of Ln3+ consists of six Cl- anions forming a slightly distorted octahedral LnCl63- center. The second coordination sphere is composed of nine BMI+ cations. The emission spectra and luminescence lifetimes of both (BMI)3LnCl6 crystals and LnCl3 in BMICI solution were measured. The spectroscopic similarities suggest that crystalline (BMI)3LnCl6 provides a good model of the Ln3+ coordination environment in BMICl solution. [ABSTRACT FROM AUTHOR]

Copyright of Inorganic Chemistry is the property of American Chemical Society and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder’s express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Chemistry Department.

Roripaugh, Lee Ann. (2014). Moist Towelette. North American Review, 299(2), 14-15.

English Department.

Flores, Jose D., & Gonzalez-Olivares, E. (2014). Dynamics of a predator-prey model with Allee effect on prey and ratio-dependent functional response. Ecological Complexity, 18, 59-66.

We analyze a ratio-dependent predator-prey model with Allee effect on the prey by making a parametric analysis of the stability properties of the dynamics of the system in which the functional response is a function of the ratio of prey to predator abundance. An important mathematical feature of these type of models is that while the functional response is undefined at the origin, the origin is singular equilibrium. We present the different types of system behaviors for different parameter values, showing the existence of separatrix curves in the phase plane determining that the long-term system’s dynamic is dependent on the initial conditions. The model is studied analytically as well as numerically, including stability and bifurcation analysis. We also discuss the biological relevance of the model regarding both coexistence (conservation) and extinction (biological control) issues. (C) 2014 Elsevier B.V. All rights reserved.

Mathematics Department.

Mirabolfathi, N., Amman, M., Faiez, D., Luke, P. N., Martin, Ryan D., Rolla, J. A., . . . Vetter, K. (2014). Neganov-Luke Phonon Amplification in P-type Point Contact Detectors. Journal of Low Temperature Physics, 176(3-4), 209-215.

The Cryogenic Dark Matter Search (CDMS) detectors measure ionization and athermal phonons in high purity germanium crystals to discriminate between nuclear recoils from dark matter candidates and radioactive backgrounds. In order to reach lower energy detection thresholds, the CDMSlite experiment operates the CDMS detectors with a larger voltage bias to increase the signal-to-noise ratio using the Neganov-Luke effect. Breakdown in those detectors was observed at fields of order 30 V/cm, but the reason for the breakdown is unknown. It is unclear if the breakdowns are due to surface leakage current, impact ionization in the bulk of the crystals, or some other effect due to the very low operating temperatures of the detectors. Germanium detectors used in gamma spectroscopy at 77 K are regularly operated with fields in excess of 1,000 V/cm. In order to understand the origin of breakdown in the CDMS detectors, a P-type Point Contact detector was equipped with transition edge phonon thermistors and operated at a base temperature of 30 mK. The linearity of the Neganov-Luke phonon amplification was studied and no sign of breakdown for biases up to 400 V was observed. This excludes impact ionization on neutral impurity states as the primary cause of the breakdown observed in the CDMSLite detectors. This demonstrates that the Neganov-Luke phonon amplification is a viable method for lowering the energy threshold in germanium detectors of masses of order 1 kg.

Physics Department.

Emery, Noah N., Simons, Jeffrey S., Clarke, C. Joseph, & Gaher, Raluca M. (2014). Emotion differentiation and alcohol-related problems: The mediating role of urgency. Addictive Behaviors, 39(10), 1459-1463.

Deficits in emotional and behavioral regulation figure prominently in etiological models of alcohol-related problems. This study tests a model linking poor differentiation of emotion to alcohol-related problems via urgency. The sample consisted of 102 undergraduates between the ages 18 and 24 who reported moderate to heavy alcohol consumption. As hypothesized, negative urgency mediated the relationship between negative emotion differentiation and alcohol-related problems. However, contrary to hypothesis, positive urgency was not associated with either positive emotion differentiation or alcohol-related problems and the indirect effect of positive emotion differentiation via positive urgency was not significant. Instead, positive emotion differentiation exhibited a significant direct effect on alcohol-related problems. This study provides an initial examination of connections between specificity in labeling emotions, behavioral disinhibition, and problematic alcohol use. These findings suggest that poor differentiation of negative emotion may foster impulsive behavior when negatively aroused. Whereas, impulsive behavior when positively aroused may reflect heightened sensitivity to positive reinforcement, which may not be related to reflective processes underlying emotion differentiation.

Psychology Department.

Struckman-Johnson, Cindy, Gaster, Samuel, & Struckman-Johnson, Dave. (2014). A preliminary study of sexual activity as a distraction for young drivers. Accident Analysis& Prevention, 71, 120-128.

In what may be the first in-depth study of sexual activity as a driving distraction in the US, a sample of 195 male and 511 female college students at a Midwestern university (M age = 19.7) participated in an on-line study of sex while driving (SWD). Of these, 64 (32.8%) men and 47 (9.3%) women had engaged in sex while driving (SWD). Nine percent of men and 29% of women had engaged in SWD as a passenger. In most recent SWD incidents, respondents reported that the two most common acts were oral sex (70.3%) and genital touching (60.4%). About 11% engaged in vaginal intercourse. Sexual activity lasted from 1 to 10 min for 42.7% of the respondents. Nearly half (49.1%) were traveling 61-80 mph during sex. Considering respondents’ lifetime incidents of SWD, the most common driving errors reported were speeding (37.8%), drifting into another lane (36%), and letting go of the steering wheel (10.8%). Only 1.8% nearly had a crash, and none actually had a crash. Separate regression analyses for male and female respondents revealed that lower intentions to engage in SWD in the future were associated with higher estimates of the probability of a car crash. The authors consider SWD to be an under-reported in-vehicle distraction and encourage more research and prevention efforts.

Psychology Department.

Ahrendt, April D., Kattelmann, Kendra K., Rector, Thomas S., & Maddox, David A. (2014). The effectiveness of telemedicine for weight management in the MOVE! Program. The Journal of Rural Health, 30(1), 113-119.

Purpose: To examine the effectiveness of videoconferencing technology for delivering comprehensive weight management treatment. Methods: This retrospective cohort study was conducted by extraction of data from medical records for the years 2008‐2010. The treatment included a series of 12 weekly MOVE!® classes delivered using videoconferencing. Data were extracted from the time of baseline weight to 1 year after baseline weight for the MOVE! participants (n = 60) and from a concurrent control group (n = 60) that did not participate in MOVE! treatment. Findings: Results indicated that the MOVE! group lost weight while the control group gained weight, resulting in a mean difference between the groups of −5.5 ± 2.7 kg (95% CI = −8.0 to −3.0; P < .0001). Conclusions: These results indicate that videoconferencing is an effective method to provide the MOVE! Weight Management Program to veterans. Weight loss was maintained for one year after baseline in the MOVE! group. This is very promising as weight re‐gain is a common issue and these results support using videoconferencing for a long‐term weight management treatment option. (PsycINFO Database Record (c) 2014 APA, all rights reserved). (journal abstract)

Sanford School of Medicine, Sioux Falls Campus.

Harris, William S. (2014). Achieving optimal n-3 fatty acid status: the vegetarian’s challenge … or not. American Journal of Clinical Nutrition, 100(1), 449S-452S.

The long chain n-3 (omega-3) fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), although originally synthesized by microorganisms in the oceans, are primarily obtained from the consumption of fish. Vegetarians, by definition, do not eat fish and thus consume virtually no EPA and DHA. Because conversion of the plant-derived n-3 fatty acid a-linolenic acid (ALA) to EPA and DHA is very low, n-3 tissue concentrations in vegetarians are lower than in omnivores. This review asks 2 questions: what is the evidence that increased n-3 concentrations reduce the risk of cardiovascular disease in vegetarians, and, if it does, how can vegetarians increase their blood and tissue concentrations of these animal-derived fatty acids? At present, both cardiovascular risk markers and cardiovascular events appear to be significantly reduced in vegetarians compared with those in omnivores. If so, and in the absence of data to show that risk in vegetarians could be even lower with higher n-3 concentrations, then the second question becomes moot. However, the absence of evidence is not evidence of absence; therefore, at our present state of knowledge, increasing n-3 concentrations is not an unreasonable goal for vegetarians. This can be accomplished by a variety of approaches, including increased intakes of ALA, consumption of stearidonic acid enriched soybean oil (if and when it comes to the market), and the use of supplements containing EPA, DHA, or both derived from nonanimal sources (microalgae, biotech yeast, and, in the future, biotech plant oils).

Sanford School of Medicine, Sioux Falls Campus.

Hershman, D. L., Lacchetti, C., Dworkin, R. H., Smith, E. M. L., Bleeker, Jonathan, Cavaletti, G., . . . Loprinzi, C. L. (2014). Prevention and Management of Chemotherapy-Induced Peripheral Neuropathy in Survivors of Adult Cancers: American Society of Clinical Oncology Clinical Practice Guideline. Journal of Clinical Oncology, 32(18), 1941-+.

Purpose To provide evidence-based guidance on the optimum prevention and treatment approaches in the management of chemotherapy-induced peripheral neuropathies (CIPN) in adult cancer survivors. Methods A systematic literature search identified relevant, randomized controlled trials (RCTs) for the treatment of CIPN. Primary outcomes included incidence and severity of neuropathy as measured by neurophysiologic changes, patient-reported outcomes, and quality of life. Results A total of 48 RCTs met eligibility criteria and comprise the evidentiary basis for the recommendations. Trials tended to be small and heterogeneous, many with insufficient sample sizes to detect clinically important differences in outcomes. Primary outcomes varied across the trials, and in most cases, studies were not directly comparable because of different outcomes, measurements, and instruments used at different time points. The strength of the recommendations is based on the quality, amount, and consistency of the evidence and the balance between benefits and harms. Recommendations: On the basis of the paucity of high-quality, consistent evidence, there are no agents recommended for the prevention of CIPN. With regard to the treatment of existing CIPN, the best available data support a moderate recommendation for treatment with duloxetine. Although the CIPN trials are inconclusive regarding tricyclic antidepressants (such as nortriptyline), gabapentin, and a compounded topical gel containing baclofen, amitriptyline HCL, and ketamine, these agents may be offered on the basis of data supporting their utility in other neuropathic pain conditions given the limited other CIPN treatment options. Further research on these agents is warranted. (C) 2014 by American Society of Clinical Oncology

Sanford School of Medicine, Sioux Falls Campus.

Kim, D. I., Birendra, K. C., Zhu, W. H., Motamedchaboki, K., Doye, V., & Roux, Kyle J. (2014). Probing nuclear pore complex architecture with proximity-dependent biotinylation. Proceedings of the National Academy of Sciences of the United States of America, 111(24), E2453-E2461.

Proximity-dependent biotin identification (BioID) is a method for identifying protein associations that occur in vivo. By fusing a promiscuous biotin ligase to a protein of interest expressed in living cells, BioID permits the labeling of proximate proteins during a defined labeling period. In this study we used BioID to study the human nuclear pore complex (NPC), one of the largest macromolecular assemblies in eukaryotes. Anchored within the nuclear envelope, NPCs mediate the nucleocytoplasmic trafficking of numerous cellular components. We applied BioID to constituents of the Nup107-160 complex and the Nup93 complex, two conserved NPC subcomplexes. A strikingly different set of NPC constituents was detected depending on the position of these BioID-fusion proteins within the NPC. By applying BioID to several constituents located throughout the extremely stable Nup107-160 subcomplex, we refined our understanding of this highly conserved subcomplex, in part by demonstrating a direct interaction of Nup43 with Nup85. Furthermore, by using the extremely stable Nup107-160 structure as a molecular ruler, we defined the practical labeling radius of BioID. These studies further our understanding of human NPC organization and demonstrate that BioID is a valuable tool for exploring the constituency and organization of large protein assemblies in living cells.

Sanford School of Medicine, Sioux Falls Campus.

Levin, Sondra W., Baker, Eva H., Zein, Wadih M., …., Griffin, Kurt J., Bianconi, Simona, . . . Mukherjee, Anil B. (2014). Oral cysteamine bitartrate and N-acetylcysteine for patients with infantile neuronal ceroid lipofuscinosis: a pilot study. Lancet Neurology, 13(8), 777-787.

Background: Infantile neuronal ceroid lipofuscinosis is a devastating neurodegenerative lysosomal storage disease caused by mutations in the gene (CLN1 or PPT1) encoding palmitoyl-protein thioesterase-1 (PPT1). We have previously reported that phosphocysteamine and N-acetylcysteine mediate ceroid depletion in cultured cells from patients with this disease. We aimed to assess whether combination of oral cysteamine bitartrate and N-acetylcysteine is beneficial for patients with neuronal ceroid lipofuscinosis. Methods: Children between 6 months and 3 years of age with infantile neuronal ceroid lipofuscinosis with any two of the seven most lethal PPT1 mutations were eligible for inclusion in this pilot study. All patients were recruited from physician referrals. Patients received oral cysteamine bitartrate (60 mg/kg per day) and N-acetylcysteine (60 mg/kg per day) and were assessed every 6-12 months until they had an isoelectric electroencephalogram (EEG, attesting to a vegetative state) or were too ill to travel. Patients were also assessed by electroretinography, brain MRI and magnetic resonance spectroscopy (MRS), and electron microscopic analyses of leukocytes for granular osmiophilic deposits (GRODs). Children also underwent physical and neurodevelopmental assessments on the Denver scale. Outcomes were compared with the reported natural history of infantile neuronal ceroid lipofuscinosis and that of affected older siblings. This trial is registered with, number NCT00028262. Findings: Between March 14, 2001, and June 30, 2012, we recruited ten children with infantile neuronal ceroid lipofuscinosis; one child was lost to follow-up after the first visit and nine patients (five girls and four boys) were followed up for 8 to 75 months. MRI showed abnormalities similar to those in previous reports; brain volume and N-acetyl aspartic acid (NAA) decreased steadily, but no published quantitative MRI or MRS studies were available for comparison. None of the children acquired new developmental skills, and their retinal function decreased progressively. Average time to isoelectric EEG (52 months, SD 13) was longer than reported previously (36 months). At the first follow-up visit, peripheral leukocytes in all nine patients showed virtually complete depletion of GRODs. Parents and physicians reported less irritability, improved alertness, or both in seven patients. No treatment-related adverse events occurred apart from mild gastrointestinal discomfort in two patients, which disappeared when liquid cysteamine bitartrate was replaced with capsules. Interpretation: Our findings suggest that combination therapy with cysteamine bitartrate and N-acetylcysteine is associated with delay of isoelectric EEG, depletion of GRODs, and subjective benefits as reported by parents and physicians. Our systematic and quantitative report of the natural history of patients with infantile neuronal ceroid lipofuscinosis provides a guide for future assessment of experimental therapies. Funding: National Institutes of Health.

Sanford School of Medicine, Sioux Falls Campus.

May, P. A., Hamrick, K. J., Corbin, K. D., Hoyme, H. Eugene, Hasken, J. M., Marais, A. S., Brooke, L. E., . . . Gossage, J. P. (2014). Dietary intake, nutrition, and fetal alcohol spectrum disorders in the Western Cape Province of South Africa. Reproductive Toxicology, 46, 31-39.

In this study, we describe the nutritional status of women from a South African community with very high rates of fetal alcohol spectrum disorders (FASD). Nutrient intake (24-h recall) of mothers of children with FASD was compared to mothers of normal controls. Nutrient adequacy was assessed using Dietary Reference Intakes (DRIs). More than 50% of all mothers were below the Estimated Average Requirement (EAR) for vitamins A, D, E, and C, thiamin, riboflavin, vitamin B-6, folate, calcium, magnesium, iron, and zinc. Mean intakes were below the Adequate Intake (AI) for vitamin K, potassium, and choline. Mothers of children with FASD reported significantly lower intake of calcium, docosapentaenoic acid (DPA), riboflavin, and choline than controls. Lower intake of multiple key nutrients correlates significantly with heavy drinking. Poor diet quality and multiple nutritional inadequacies coupled with prenatal alcohol exposure may increase the risk for FASD in this population. (C) 2014 Elsevier Inc. All rights reserved.

Sanford School of Medicine, Sioux Falls Campus.

Persons, J. E., Robinson, J. G., Ammann, E. M., …., Harris, William S., Manson, J. E., & Fiedorowicz, J. G. (2014). Omega-3 fatty acid biomarkers and subsequent depressive symptoms. International Journal of Geriatric Psychiatry, 29(7), 747-757.

ObjectiveWe sought to determine the relationship between the omega-3 fatty acid content of red blood cell membranes (RBC), in particular docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), and baseline and new-onset depressive symptoms in post-menopausal women. We secondarily sought to characterize the association between dietary omega-3 fatty acid intake and depressive symptomatology. MethodsStudy participants included 7086 members of the Women’s Health Initiative Memory Study (aged 63-81years) who had an assessment of RBC omega-3 fatty acid concentrations at the baseline screening visit. Depressive symptoms at baseline and follow-up were characterized using the Burnam eight-item scale for depressive disorders (Center for Epidemiologic Studies Depression Scale/Diagnostic Interview Schedule short form) and secondarily additionally inferred by antidepressant medication use. ResultsIn multivariable-adjusted models, our primary exposure, RBC DHA+EPA, was not related to depressive symptoms by any measure at baseline or follow-up, nor were RBC total omega-3, DHA, or EPA (all p>0.2). In contrast, dietary intake of omega-3 was positively associated with depressive symptoms at baseline (adjusted odds ratio 1.082, 95% confidence interval 1.004-1.166; p=0.04 for dietary DHA+EPA and Burnam score 0.06), although this generally did not persist at follow-up. ConclusionNo relationship between RBC omega-3 levels and subsequent depressive symptoms was evident, and associations between dietary omega-3 and depressive symptoms were variable. Biomarkers of omega-3 status do not appear to be related to risk of new depression in post-menopausal women.

Sanford School of Medicine, Sioux Falls Campus.

Reindl, Bailey A., Lynch, Douglas W., & Jassim, Ali D. (2014). Aggressive Variant of a Solid Pseudopapillary Neoplasm: A Case Report and Literature Review. 138, 974-978.

Solid pseudopapillary neoplasm, a lesion of uncertain cellular differentiation, is an unusual tumor of the pancreas with an indolent clinical course that typically arises in young females. We report a case of solid pseudopapillary neoplasm arising in a 17-year-old adolescent girl who presented with progressive abdominal pain. The patient underwent surgical resection of an 18 × 14 × 8-cm pancreatic mass that displayed the usual histologic features of a solid pseudopapillary neoplasm in addition to prominent nuclear atypia, increased proliferative index, and extensive necrosis. These unusual histologic findings are rare and are of particular interest owing to the dramatically decreased survival time displayed in this case. Although precise pathologic criteria suggesting a high risk for aggressive behavior of solid pseudopapillary neoplasms are uncertain, recognition of the unusual pathologic features displayed in this case may be useful in the prediction of potentially more aggressive neoplasms that portend a poorer prognosis.

Sanford School of Medicine, Sioux Falls Campus.

Terrier, B., Nagata, Satashi, Ise, Tomoko, Rosenzwajg, M., Pastan, I., Klatzmann, D., . . . Cacoub, P. (2014). CD21(-/low) Marginal Zone B Cells Highly Express Fc Receptor-like 5 Protein and Are Killed by Anti-Fc Receptor-like 5 Immunotoxins in Hepatitis C Virus-Associated Mixed Cryoglobulinemia Vasculitis. Arthritis & Rheumatology, 66(2), 433-443.

Objective. Hepatitis C virus (HCV) is associated with B cell lymphoproliferative disorders, including mixed cryoglobulinemia (MC) vasculitis and B cell non-Hodgkin’s lymphoma. The expansion of clonal and autoreactive rheumatoid factor-bearing CD21(-/low) marginal zone (MZ) B cells was demonstrated in patients with HCV-associated MC vasculitis. Fc receptor-like (FCRL) proteins comprise a family of immunoregulatory proteins preferentially expressed on B lineage cells. The goal of this study was to investigate the expression of FCRL proteins 1-5 on B cells from patients with HCV-associated MC vasculitis. Methods. Expression of FCRL proteins 1-5 was assessed by flow cytometry on B cells from 15 HCV-infected patients with type II MC (7 of whom had B cell non-Hodgkin’s lymphoma), 20 HCV-infected patients without MC, and 20 healthy donors. To evaluate FCRL-5 as an immunotherapy target in HCV-associated MC vasculitis, 2 anti-FCRL-5 recombinant immunotoxins were produced using anti-FCRL-5 monoclonal antibodies and Pseudomonas exotoxin. Results. Expression of FCRLs 2, 3, and 5 was markedly increased while expression of FCRL-1 was decreased on clonal CD21(-/low) MZ B cells, as compared with other B cell subsets, from HCV-infected patients and healthy donors. However, there was no difference in the pattern of FCRL expression between HCV-MC patients with lymphoma and those without lymphoma. The anti-FCRL-5 immunotoxins showed specific cytotoxicity against FCRL-5-expressing clonal CD21(-/low) MZ B cells isolated from HCV-infected patients as well as FCRL-5-transfected cell lines. No cytotoxicity against T cells or conventional B cells was observed. Conclusion. These findings suggest that FCRL-5-targeting therapies could be a specific treatment for HCV-associated MC vasculitis and other FCRL-5-positive autoimmune B cell disorders.

Sanford School of Medicine, Sioux Falls Campus.

Talbot, Elizabeth Peffer, & McMillin, Joan A. (2014). The Social Work Reinvestment Initiative: Advocacy and Social Work Practice. Social Work, 59(3), 201-210.

In 2006, NASW launched the Social Work Reinvestment Initiative by granting each state chapter $15,000 in seed money to address the most pressing social work needs in the state. This article describes how NASW-SD, with 246 members, launched an epic campaign that resulted in the establishment of the only MSW program in South Dakota. Using historical research methods, this article demonstrates the power of social work advocacy when members unify in pursuit of a common goal and describes how the social workers rallied to educate policymakers and the public on the value of social work and its delivery of necessary social services at all levels and in all fields of practice. The research highlights an uphill battle of advocacy and the skillful planning and implementation of a campaign to secure state funding to establish the first MSW program in the state, at the beginning of the most difficult economic recession since the Great Depression.

School of Health Sciences.



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